Soni et al (p. 7) describe their new method of customizing TIR illumination as one that acts as a complement to the well known ion-current measurements applied in nanopore-based sensing. The ability to having optical detection working simultaneously with ion-current measurement is shown to enable cause molecules to be excited and a nanoscale pore of approximately 4 nm which is put between two fluids helps in threading image molecules. Using necessary hardware (for both electrical functions and optical capabilities), changes that occur in the ionic current are detected and this happens simultaneously with detection of fluorescence signals from labeled biomolecules.
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