The products of restriction digestion were separated on 1.0% agarose gel that had been stained with Âµg/ml 0.5ethidium bromide. First, two samples consisting of the pBKS II plasmid digest andÂ Pc-FtsZÂ insert were placed on the gel containing 5Âµl loading buffer. The nest step involved running the gel in a tank containing TAE buffer. A MW marker was loaded into the first and last wells on the gel. 20Âµl of each sample was loaded into different wells. Similarly, undigested samples were loaded into the gel to act as controls. The gel was run at 110V for 50 min to separate the fragments into distinct bands.
The bands corresponding to the plasmid and insert pattern were cut out before being transferred into a micro-centrifuge tube. Its mass was determined before adding QG buffer (three volumes). Subsequent steps involved repeated incubation, dissolution in isopropanol, and centrifugation of the samples at maximum speed to bind the samples to the spin column. The spin column was then washed in EB buffer to elute the DNA. The purified DNA was stored at -20Â°C for the next step.
Delivering a high-quality product at a reasonable price is not enough anymore.
That’s why we have developed 5 beneficial guarantees that will make your experience with our service enjoyable, easy, and safe.
You have to be 100% sure of the quality of your product to give a money-back guarantee. This describes us perfectly. Make sure that this guarantee is totally transparent.Read more
Each paper is composed from scratch, according to your instructions. It is then checked by our plagiarism-detection software. There is no gap where plagiarism could squeeze in.Read more
Thanks to our free revisions, there is no way for you to be unsatisfied. We will work on your paper until you are completely happy with the result.Read more
Your email is safe, as we store it according to international data protection rules. Your bank details are secure, as we use only reliable payment systems.Read more
By sending us your money, you buy the service we provide. Check out our terms and conditions if you prefer business talks to be laid out in official language.Read more