The inner membranes of the chloroplast contain the chlorophyll along with the other photosynthetic pigments that are involved in light energy harvesting. The inner membrane, the grana, and the thylakoids gained a lot of importance because of the presence of the primary pigments and their apparent cytological appearance. However, it was observed that the carbon reduction and fixation stopped when the chloroplast’s outer membrane was broken. Hence it was found that the stroma or the transparent aqueous matrix of the chloroplast that seemed to only simply the pigmented substances also played an essential role in the process of photosynthesis. The chloroplasts evolved from the free-living prokaryotes that established an endosymbiotic association with some of the eukaryotic cells. Therefore, the stroma of the chloroplast continues to encompass ribosomes and DNA to perform the process of protein synthesis. The proteins produced include the one’s that are essential in the photosynthesis’ light-independent reactions and also the reactions that occur to fix the inorganic minerals like the nitrates in organic molecules.
The function of the chloroplast stroma
The chloroplast is the organelle of the plant that contains its own genome as well as performs the most crucial activity of the cell. Many genes that are important for the function of the chloroplast have also been incorporated into its nuclear genome. Therefore, the chloroplast should be able to adjust its metabolic activity so that it can complement the working of the cell. In the chloroplast, the stroma is found to be very important for this since it contains the enzymes that are required for the process of carbon fixation and it also manages the response of the chloroplast to cellular signaling that occurs between the various organelles and the cellular stresses. The stroma plays a crucial role in the light-independent reactions as well as the light-dependent reactions of photosynthesis. When there is extreme stress, the stroma of the chloroplast can selectively permit autophagy without eliminating the pigment molecules and the inner membranous structures. The finger-like protrusions that occur from the stroma, and do not include any thylakoids are observed to be closely attributed to the endoplasmic reticulum and the nucleus, and it contributes to many sophisticated regulatory mechanisms.
Thylakoids
The inner membrane of the chloroplast encloses the fluid-filled region known as the stroma that encompasses enzymes that are required for the photosynthesis’ light-independent reactions. This inner membrane in folds and it forms the interconnected stacks of thylakoids that are known as the disk-like sacs, and they are always arranged in stacks known as the grana. The thylakoid system is found to be suspended in the chloroplast’s stroma. The thylakoids or the membranous sacks are arranged in stacks. Each granum of the chloroplast comprises of around 10-20 thylakoids. The fluid-filled interior space of the thylakoid is enclosed by the thylakoid membrane. The chlorophyll, other photosynthetic pigments, and also the electron transport chains are present within the thylakoid membrane. The photosynthesis’ light-dependent reactions take place in the thylakoids. Chlorophyll is the photosynthesis’s primary pigment and or absorbs the light in the visible light spectrum’s red and blue region and the green light gets reflected. Chlorophyll A and Chlorophyll B are the two major kinds of chlorophylls. The thylakoid membranes can also encompass some other accessory pigments such as the carotenoids and the phycocyanin’s. The light energy is absorbed by these accessory pigments and it is transferred to the chlorophyll.
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