Restriction enzymes act at the restriction sites of single or double-stranded DNA (Pingoud, Alves & Geiger, 1993; Tovkach Zeevi & Tzfira 2010), and cut them at these sites.
To facilitate digestion, 10 Âµl of Dna was added. In addition, 2 Âµl of BSA was added. 2 Âµl of buffer was also added, and a further 1 Âµl retraction enzyme added. To this was added 5 Âµl Sterile Water, and all things were put in an epp tube.
The digestion process involved the addition of 10 Âµl of Dna, 2 Âµl of BSA, 2 Âµl of buffer, and 6 Âµl Sterile Water.Everything was then placed in an epp tube. The digest was run, and then taken on a gel agroass.
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