To the PBS were added protein inhibitors. Thereafter 200 ml pellet was resuspended in 1oml PBS with 100 μl lysozyme enzyme. The contents were then incubated at room temperature for 15 minutes on the rotary suspension mixer. Thereafter, the contents were freeze-thawed 7 times, first using liquid nitrogen, then in a water bath, at 37 oC. Then, 100 μl DNase enzymes were added, and the contents incubated on the rotary suspension mixer for 15 minutes. Spinning then followed at 12,000rpm for 30 min, at a speed of 4oC. The supernatant and the pellet were then placed in separate tubes and the sample loaded on SDS page.
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