â€œGene amplification and PCR provide species-specific detection with high sensitivity, though the process is arduous, as it requires the extraction of DNA or RNA from microbial cellsâ€ (Yamaguchi, Ohba and Nasu 631). According to Yamaguchi, Ohba, and Nasu, flow cytometry is used in the rapid identification of bacteria in milk through methods such as â€œtotal bacterial counts, detection of pseudomonas cells by fluorescence in situ hybridization, and detection of respiring Escherichia coli O157:H7 cells by double staining with Fluorescein Isothiocyanate -labeled antibody and 5-cyano-2, 3-ditolyl tetrazolium chlorideâ€. Though the flow cytometry technique is fast, responsive, and consistent, the equipment is expensive, and its operation is complex for the untrained personnel (Yamaguchi, Ohba, and Nasu 632)
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