The test item was ten rats, five male five females, from the same species. The rats were young healthy adults that were not older than 9weeks. The females were not pregnant to ensure that they did not affect the results. On weight, it was ensured that it was not more or less than 20% of the mean (male and female).since there were planned interim kills, the number of rats was increased by ten more, five males and five female.
Dose levels were selected taking into account kinetic toxicity. The highest dose level was chosen to induce toxic effects. Thereafter, a reducing sequence of dose levels was selected in order to show any dose related response and.two, to four fold intervals were used for descending dose levels. A fourth test group was used between dosages.
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