The chip-based method of detecting bacteria in milk requires the milk to be cleared, in order to avoid â€œfalse-positive results due to nonspecific binding of fluorescent dyes and probes to milk compoundsâ€ (Yamaguchi, Ohba and Nasu 633). Another way in which negative results can be obtained is interference during fluorescent staining and FISH. The system was designed for simple flow cytometry, and requires no training on operation, as it is also used in identification of bacterial cells in both culture and natural river water samples. The results are indicated as dot plots of the fluorescence values and the specificity of the optimized FISH procedure are proven using the total direct counting method (Yamaguchi, Ohba and Nasu 633).
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