The purpose of this practical assignment was based on the identification of auxotrophic requirements in bacteria and antibiotic resistance in bacteria, testing of mutagens and effects of Ultraviolet light on mutagens. The experiments were conducted separately under four different objectives. It was found out that methionine, as nutrient is required for bacterial growth. E.coli bacteria were found to be resistant to tetracycline. In addition, cigarette was found to be carcinogenic and exposure to UV light does not kill cell when the duration is below ten minutes.
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